As with other alkylating agents in the nitrosourea class, it is toxic to cells by causing damage to the dna, though other mechanisms may also contribute. The full text of this article is available as a pdf 180k. The streptozotocindiabetic rat as a model of the chronic complications of human diabetes michael wei1 bsc, leslie ong1 bsc, maree t. Data sources include ibm watson micromedex updated 28 feb 2020, cerner multum updated 2 mar 2020. Stz is a nitrosurea compound derived from streptomyces achromogenes, which has also been used as an antibiotic and a cancer treatment. Diabetes induced by streptozotocin at 150 mgkg body weight is complete and permanent. This material is provided for educational purposes only and is not intended for medical advice, diagnosis or treatment. Stz is toxic to the insulinproducing beta cells of the pancreas and used to induce a diabetes similar to a type i. Please visit guidelines on use of streptozotocin in rodents to locate the document you need. Autoxidation of dialuric acid generates superoxide radicals. Unlike type 1 diabetes, type 2 diabetes t2d is associated with resistance to insulin action. The mechanism of alloxan and streptozotocin action in b. Reagentmaterial quantity required vendor stock numberstzna. Rodent models of streptozotocin induced diabetic nephropathy.
Streptozotocininduced diabetic ratsmice stz model advantages. The streptozotocininduced diabetes model has been extensively used, making it particularly useful for building upon and comparing results of other studies. Whatever the protocol, whatever the degree of beta cell loss, stz alone induces. The diabetesinducing agent streptozotocin stz is a.
Review article modeling type 2 diabetes in rats using high. Pdf induction of diabetes by streptozotocin in rats. Animals models for studying diabetes mellitus etuk, e. Protocol reagent preparation nacitrate buffer streptozotocin stz stznacitrate solution summary. The mechanisms of alloxan and streptozotocininduced diabetes. Antidiabetic effects of hesperidin and naringin in type 2 diabetic rats osama m. The rat mouse model of diabetes mellitus induced by streptozotocin injections is one of the most widely used model of type i diabetes and mimics human pathological situation of decreased insulin production untreated type i diabetes or decrease tissue responsiveness to insulin. Full text streptozotocininduced type 1 diabetes in.
The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and cpeptide in serum, between normal and diabetic rats. Streptozotocin can selectively destroy the pancreatic. Streptozotocin diabetes and insulin resistance impairment of spermatogenesis in adult rat testis. Part of the progress in drug research book series pdr, volume 71. Health hazards streptozotocin is an antibiotic that is produced by stretomyces achromogenes, it is a toxic and carcinogenic material. The document you are looking for is no longer available at this address. The males of various strains are differentially susceptible to developing diabetes.
Effect of streptozotocin diabetes and insulin treatment on. Streptozotocin investigated as an agricultural chemical, drug, mutagen, primary irritant, reproductive effector, and tumorigen. Detailed in this unit are protocols for producing stz. Standard operating procedure for streptozotocin stz in animals 1.
Streptozotocin is used to manage malignant insulinoma. The present study designed to investigate the antioxidant activity of ethanolic extract of a. Group 4 was injected with streptozotocin and later treated in the same way as in group 3 streptozotocinmanganese group. Dna damage induces activation of parp which is likely more important for diabetes induction than the dna damage itself. There are various protocols for inducing diabetes with streptozotocin furman, 2015. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water. Diabetes caused a slowing of motor and sensory nerve conduction velocity, thermal hypoalgesia, reduction in intraepidermal nerve fiber density. A new protocol of type 2 diabetes induction and hydrocortisone effects on. To develop the corresponding animal pathology model, high fat diet hfd is often used. This area will include a brief description of what the protocol is used for and why someone would need to use it. Stzinduced diabetes can be highly variable, and no standard protocol exists for the prepa ration, dose or administration of stz. Given the high morbidity rate after pancreatectomy, streptozotocin should be the method of choice for inducing diabetes mellitus in pigs. Effect of streptozotocin diabetes and insulin treatment on the rate of protein synthesis in tissues of the rat in vviuo received for publication, august 6, 1973 virginia m.
Guidelines on use of streptozotocin in rodents ulam. How to induce permanent diabetes by streptozotocin in. Highfat diet and streptozotocin in the induction of type 2 diabetes. This model can be established in genetically modified rodents for investigating the role of molecular mechanisms and genetic susceptibility in the development of diabetic nephropathy. Streptozotocin stz is commonly used to induce experimental diabetes in animals. We sought to determine the effect of dipeptidyl peptidase iv dppiv inhibition on streptozotocin diabetesinduced vascular and neural dysfunction. Streptozotocin stz is an antibiotic that produces pancreatic islet cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus t1dm.
This protocol is used by amdcc members to induce diabetes in anumber of the animal models developed by the consortium. Standard operating procedure for streptozotocin stz in. Garlicky from the department of human nutrition, london school of hygiene and tropical medicine, clinical nutrition. Treatment of streptozotocininduced diabetic rats with. Food consumption was measured in terms of gm, water consumption was measured in terms of ml and urine volume was measured in terms of ml on a daily basis and glucose in blood serum were also measured, so that chemical diabetes was verified in rats injected with. Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. Streptozotocininduced type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic. Propel your preclinical research using jax inventoried, ondemand, and unique stzinjected models generated on various genetic backgrounds, as well as additional services. Hypoglycemia induced by an injection of insulin or hyperglycemia induced by. Streptozotocin stz is an alkylating agent that targets insulinproducing beta cells in the pancreas, mimicking type 1 diabetes phenotypes in mice. Lowdose streptozotocin induction protocol mouse pages 1. Mrinmay das2, ashok kumar d1, mastanaiah k2 and arup das2. Streptozotocinhigh fat dietinduced type 2 diabetes bienta.
Szkudelski department of animal physiology and biochemistry, university of agriculture, poznan, poland received november 2, 2000 accepted march 20, 2001 summary alloxan and streptozotocin are widely used to induce experimental diabetes in animals. All mice were fasted for models of iddm 47 but also niddm with 20. Streptozotocinhigh fat dietinduced type 2 diabetes. Evaluation of antidiabetic property on streptozotocininduced. Streptozotocin, which is composed of 1methyl1nitrosurea attached to dglucose, is selective for the pancreatic islet beta cell because of its glucose moiety, which allows its transport into beta cell using the glucose transporter glut2 szkudelski, 2001. Hoey4 phd, darryl burstow5 mbbs fracp and lindsay brown1 phd departments of physiology and pharmacology1 and pharmacy2, the university of queensland.
Only select registry of toxic effects of chemical substances rtecs data is presented here. Streptozotocin diabetes and insulin resistance impairment. Evaluation of antidiabetic activity of ethanolic extract. Streptozotocin use in mice and rats university of melbourne. Diabetes, highfat diet and lowdose streptozotocin, micro.
A single injection of streptozotocin into mice produced an extensive necrosis of the beta cells resulting in permanent diabetes. Streptozotocin use in mice and rats version 1 date of publication. In control and streptozotocintreated female rats, we investigated the effects of diabetes on vaginal blood flow, tissue morphology, and expression of arginase i, endothelial nitric oxide synthase enos and cgmp. Pdf diabetes mellitus represents a group of metabolic disorders affecting about. However, the effects of diabetes on genital tissue structure, innervation and function remains poorly characterized. Diabetes, high fat diet and low dose streptozotocin, micro and macrovascular complications. The aim of this paper is to describe a step by step protocol on how to induce diabetes type 2 in rats using streptozotocin stz. Streptozotocin stzinduced diabetes mellitus dm offers a very costeffective and expeditious technique that can be used in most strains of rodents, opening the field of dm research to an array of genotypic and phenotypic options that would otherwise be inaccessible. Induction of diabetes by streptozotocin in rats springerlink.
Ebscohost serves thousands of libraries with premium essays, articles and other content including induction of diabetes mellitus in rats using intraperitoneal streptozotocin. Streptozotocininduced diabetes in the rat is associated. Streptozotocin is a glucosaminenitrosourea compound. Pdf experimental model of streptozotocinnicotinamide induced. U department of pharmacology, college of health sciences, usmanu danfodiyo university. In our experiments, we followed the rats with streptozotocininduced diabetes for one year and a. Nnitroso derivative of glucosamine study protocol was approved by animal ethics committee has been commonly used to induce not only animal of jamia hamdard, new delhi. This protocol is used by diacomp members to induce diabetes in a number of the animal models developed by the consortium. Research article in streptozotocin induced diabetic rats. A new protocol of type 2 diabetes induction and hydrocortisone. Pdf a practical guide for induction of type2 diabetes in rat. O department of physiology, 1faculty of basic medical sciences, college of medicine, university of lagos, idi. Streptozotocininduced experimental diabetes in male. Fvbnj 001800, balbcj 000651, and aj 000646 males are resistant.
The mechanism of alloxan and streptozotocin action in b cells of the rat pancreas t. Streptozotocininduced diabetic models in mice and rats. Access codes and supplements are not guaranteed with used items. Shows some signs of wear, and may have some markings on the inside.
Diabetes is associated with declining sexual function in women. Stzinduced diabetes can be highly variable, and no standard protocol exists for the preparation, dose, or administration of stz. Most of the literature to date includes very little description of the technical details of diabetes induction, stzrelated moribundity and mortality rates, and severity of diabetes experienced. Streptozotocininduced type 1 diabetes in rodents as a. Induction of diabetes by streptozotocin in rats ncbi nih. This induction protocol isused to induce a diabetes similar to a type i diabetic. Nowadays, diabetes trends to spread throughout the world in both developed and developing countries. Streptozotocin stz is an alkylating agent that affects pancreatic islets, inducing diabetes in mice. Single dose streptozotocininduced diabetes sage journals. Evaluation of antidiabetic activity of ethanolic extract of alternanthera sessilis linn. The histological changes in the islets were similar to those described after the injection of alloxan. A pilot trial is recommended for use in novel conditions. Add to cart enter your mobile number or email address below and well send you a link to. To understand the pathophysiological mechanisms and factors involved in diabetes, the use of pancreatic regeneration studies is increasing in an attempt to understand the behavior of pancreatic beta cells.
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